ERCC1 Monoclonal Antibody

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Catalogue Numbers: MB65921-50, MB65921-100

Sizes: 50µl, 100µl

Swiss-Prot: P07992

Host: Mouse

Reactivity: Human

Applications: WB, IHC

Background: DNA repair systems operate in all living cells to manage a variety of DNA lesions. Nucleotide excision repair (NER) is implemented in cases where bulky helix-distorting lesions occur, such as those brought about by UV and certain chemicals. Excision Repair Cross Complementing 1 (ERCC1) forms a complex with ERCC4/XPF, which acts as the 5’ endonuclease required to excise the lesion. ERCC1-XPF is also required for repair of DNA interstrand crosslinks (ICLs) and involved in repair of double strand breaks. Research studies have shown that expression of ERCC1 is related to survival rate and response to chemotherapeutic drugs in several human cancers including non-small cell lung cancer (NSCLC).

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of ERCC1 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of ERCC1 expression in Hela (A), HepG2 (B), 293T (C), Jurkat (D) whole cell lysates., Immunohistochemical analysis of ERCC1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 36 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: DNA excision repair protein ERCC-1

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human ERCC1. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified